pCA-T-intron-G
(Plasmid #36887)

• Depositing Lab: Liqun Luo
• Publication: Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.

Backbone

• Vector backbone: pBluescript SK(+)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 5594
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: T (i.e., ATG, start codon as a first exon)
• Promoter: CAG (chicken beta actin promoter and CMV enhancer)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: see sequence
• 3′ sequencing primer: see sequence

Gene/Insert 2

• Gene/Insert name: GFP
• Alt name: mut4EGFP
• Mutation: deleted nucleotides before nucleotide 275
• Promoter: CAG (chicken beta actin promoter and CMV enhancer)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: see sequence
• 3′ sequencing primer: T3

Gene/Insert 3

• Gene/Insert name: beta-globin intron
• Mutation: insertion of a loxP site
• Promoter: CAG (chicken beta actin promoter and CMV enhancer)

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: see sequence
• 3′ sequencing primer: see sequence

Resource Information

• Supplemental Documents:
  • pCA-T-intron-G_GenBank_file
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The first exon (T) that contains the start codon is out of frame with the second GFP exon.