pGEX6P1_EHD1_438-534
(Plasmid #36459)

• Depositing Lab: Yvonne Groemping
• Publication: EH domain constructs (unpublished)

Backbone

• Vector backbone: pGEX6P1
• Backbone manufacturer: GE Healthcare
• Backbone size w/o insert (bp): 4984
• Total vector size (bp): 5284
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EHD1 EH domain
• Alt name: EH domain containing 1
• Species: H. sapiens (human)
• Insert Size (bp): 300
• Mutation: deleted amino acids 1-437 from full-length construct
• GenBank ID: NM_006795
• Entrez Gene: EHD1 (a.k.a. H-PAST, HPAST1, PAST, PAST1)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: pGEX5
• 3′ sequencing primer: pGEX3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The original clone which was used as a template was purchased from the company BioCat, Heidelberg, Germany.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry