pGEX6P1-Reps1-EH domain
(Plasmid #36860)

• Depositing Lab: Yvonne Groemping
• Publication: EH domain constructs (unpublished)

Backbone

• Vector backbone: pGEX6P1
• Backbone manufacturer: GE Healthcare
• Backbone size w/o insert (bp): 4984
• Total vector size (bp): 5321
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Reps1
• Alt name: Ral BP1 associated eps domain containing protein 1
• Alt name: (amino acids 226-314)
• Species: H. sapiens (human)
• Insert Size (bp): 277
• Mutation: deleted amino acids 1-225 and 315-769
• GenBank ID: NM_001128617
• Entrez Gene: REPS1 (a.k.a. RALBP1)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: pGEX5
• 3′ sequencing primer: pGEX3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Template was purchased from BioCat, Heidelberg, Germany

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry