pmTurquoise2-Mito
(Plasmid #36208)

• Purpose: In vivo visualization of the mitochondria (can be used for colocalization studies)
• Depositing Lab: Dorus Gadella
• Publication: Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1038/ncomms1738.

Backbone

• Vector backbone: pmTurquoise2-C1
• Total vector size (bp): 4823
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MTS
• Alt name: Mitochondrial Targeting Signal
• Alt name: COX8A(1-29)
• Species: H. sapiens (human)
• Insert Size (bp): 89
• GenBank ID: NM_004074
• Entrez Gene: COX8A (a.k.a. COX, COX8, COX8-2, COX8L, MC4DN15, VIII, VIII-L)
• Promoter: CMV
• Tag / Fusion Protein:
  • mTurquoise2 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: AGGTCTATATAAGCAGAGC
• 3′ sequencing primer: TCTACAAATGTGGTATGGC

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 20 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene's quality control sequence with EGFP-C primer shows that there is a single base change downstream of the mTurquoise in the SV40 terminator that eliminates the MluI site. Upstream of the mTurquoise, our sequence with EGFP-N primer shows a small insertion adding several additional restriction sites. These changes are in the cloning/junction region and should not affect plasmid function.