pmTurquoise2-H2A
(Plasmid #36207)

• Purpose: In vivo visualization of the nucleus (can be used for colocalization studies)
• Depositing Lab: Dorus Gadella
• Publication: Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1038/ncomms1738.

Backbone

• Vector backbone: pmTurquoise2-C1
• Total vector size (bp): 5099
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: H2A
• Alt name: Histone 2A
• Species: H. sapiens (human)
• Insert Size (bp): 393
• GenBank ID: NM_003511
• Entrez Gene: H2AC16 (a.k.a. H2A.i, H2A/i, H2AC11, H2AC13, H2AC15, H2AC17, H2AFI, HIST1H2AL, dJ193B12.9)
• Promoter: CMV
• Tag / Fusion Protein:
  • mTurquoise2 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsrgI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: AGGTCTATATAAGCAGAGC
• 3′ sequencing primer: TCTACAAATGTGGTATGGC

Resource Information

• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there is a 3bp mismatch between Addgene's quality control sequence and the provided sequence from the depositing lab. This mismatch should not affect function or localization.