pmTurquoise2-NES
(Plasmid #36206)

• Purpose: In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)
• Depositing Lab: Dorus Gadella
• Publication: Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1038/ncomms1738.

Backbone

• Vector backbone: pmTurquoise2-N1
• Total vector size (bp): 4778
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NES
• Alt name: Nuclear Export Sequence
• Species: Synthetic
• Insert Size (bp): 42
• Promoter: CMV
• Tag / Fusion Protein:
  • mTurquoise2 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsrgI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: AGGTCTATATAAGCAGAGC
• 3′ sequencing primer: TCTACAAATGTGGTATGGC

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry