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Addgene

pmTurquoise2-Golgi
(Plasmid #36205)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 36205 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmTurquoise2-N1
  • Total vector size (bp) 4904
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Golgi targeting sequence
  • Alt name
    B4GALT1(1-61)
  • Alt name
    beta4Gal-T1(1-61)
  • Alt name
    beta-1,4-galactosyltransferase 1(1-61)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    183
  • GenBank ID
    NM_001497
  • Entrez Gene
    B4GALT1 (a.k.a. B4GAL-T1, CDG2D, GGTB2, GT1, GTB, beta4Gal-T1)
  • Promoter CMV
  • Tag / Fusion Protein
    • mTurquoise2 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer AGGTCTATATAAGCAGAGC
  • 3′ sequencing primer TCTACAAATGTGGTATGGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmTurquoise2-Golgi was a gift from Dorus Gadella (Addgene plasmid # 36205 ; http://n2t.net/addgene:36205 ; RRID:Addgene_36205)

  • For your References section:

    Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%. Goedhart J, von Stetten D, Noirclerc-Savoye M, Lelimousin M, Joosen L, Hink MA, van Weeren L, Gadella TW Jr, Royant A. Nat Commun. 2012 Mar 20;3:751. doi: 10.1038/ncomms1738. 10.1038/ncomms1738 PubMed 22434194
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