GRLF1
(Plasmid #36195)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: SGC Q3&Q4 2011 (unpublished)

Backbone

• Vector backbone: pET28-MHL (Addgene plasmid 26096)
• Backbone manufacturer: SGC
• Backbone size w/o insert (bp): 7325
• Total vector size (bp): 8036
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: GRLF1 (Codon Devices Synthesized)
• Alt name: PDB: 3C5H
• Alt name: glucocorticoid receptor DNA binding factor 1 isoform b
• Alt name: GRF-1, KIAA1722, MGC10745, P190-A, P190A, p190RhoGAP
• Species: H. sapiens (human)
• Insert Size (bp): 711
• Mutation: Contains the Ras homolog domain only (amino acid residues 13-249)
• Entrez Gene: ARHGAP35 (a.k.a. GRF-1, GRLF1, P190-A, P190A, p190ARhoGAP, p190RhoGAP)
• Tag / Fusion Protein:
  • His tag with TEV cleavage (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB: 3C5H. http://www.thesgc.org/structures/3C5H/