CD200CD4d3+4-His
(Plasmid #36152)

• Purpose: A positive control receptor bait with His-tag (rat CD4d3+4-HIS) and prey (rat CD200) EXPRESs plasmid
• Depositing Lab: Gavin Wright
• Publication: Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub 2008 Feb 22.

Backbone

• Vector backbone: pTT3
• Backbone manufacturer: Yves Durocher, NRC
• Backbone size w/o insert (bp): 6000
• Total vector size (bp): 7260
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CD200
• Alt name: OX-2
• Species: R. norvegicus (rat)
• Insert Size (bp): 714
• Mutation: G2A
• Entrez Gene: Cd200 (a.k.a. Cspmo2, MRCOX2, Mox2)
• Promoter: CMV
• Tags / Fusion Proteins:
  • ratCD4d3+4 (C terminal on backbone)
  • His tag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains an intentional G2A mutation in the CD200 sequence to create a consensus Kozak sequence