mcherry-PH
(Plasmid #36075)

• Depositing Lab: Narasimhan Gautam
• Publication: Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PH domain of PLCdelta1
• Species: H. sapiens (human)
• Insert Size (bp): 510
• GenBank ID: NM_001130964
• Entrez Gene: PLCD1 (a.k.a. NDNC3, PLC-III)
• Promoter: CMV
• Tag / Fusion Protein:
  • mcherry (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7 primer
• 3′ sequencing primer: BGH reverse primer

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry