pEGFP-N1- Ncad-GFP
(Plasmid
#35676)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 35676 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4733
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNcad
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SpeciesD. rerio (zebrafish)
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Entrez Genecdh2 (a.k.a. bib, glo, lyr, ncad, pac, wu:fb47h04)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site AgeI (not destroyed)
- 5′ sequencing primer CMV fwd
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-N1- Ncad-GFP was a gift from James Jontes (Addgene plasmid # 35676 ; http://n2t.net/addgene:35676 ; RRID:Addgene_35676) -
For your References section:
Protocadherin-19 and N-cadherin interact to control cell movements during anterior neurulation. Biswas S, Emond MR, Jontes JD. J Cell Biol. 2010 Nov 29;191(5):1029-41. 10.1083/jcb.201007008 PubMed 21115806