pGEX 4T1 ERbeta(1-255)
(Plasmid
#35564)
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 35564 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepGEX 4T1
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameEstrogen Receptor beta
-
SpeciesH. sapiens (human)
-
Insert Size (bp)765
-
Mutationresidues 1-255
-
Entrez GeneESR2 (a.k.a. ER-BETA, ESR-BETA, ESRB, ESTRB, Erb, NR3A2, ODG8)
-
Tag
/ Fusion Protein
- GST (N terminal on backbone)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pGEX 4T1 ERbeta(1-255) was a gift from Harish Srinivas (Addgene plasmid # 35564 ; http://n2t.net/addgene:35564 ; RRID:Addgene_35564) -
For your References section:
Ligand-independent antiapoptotic function of estrogen receptor-beta in lung cancer cells. Zhang G, Yanamala N, Lathrop KL, Zhang L, Klein-Seetharaman J, Srinivas H. Mol Endocrinol. 2010 Sep;24(9):1737-47. Epub 2010 Jul 21. 10.1210/me.2010-0125 PubMed 20660297