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Addgene

pGEX 4T1 ERbeta
(Plasmid #35563)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35563 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX 4T1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Estrogen Receptor beta
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1592
  • Entrez Gene
    ESR2 (a.k.a. ER-BETA, ESR-BETA, ESRB, ESTRB, Erb, NR3A2, ODG8)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer NA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX 4T1 ERbeta was a gift from Harish Srinivas (Addgene plasmid # 35563 ; http://n2t.net/addgene:35563 ; RRID:Addgene_35563)

  • For your References section:

    Ligand-independent antiapoptotic function of estrogen receptor-beta in lung cancer cells. Zhang G, Yanamala N, Lathrop KL, Zhang L, Klein-Seetharaman J, Srinivas H. Mol Endocrinol. 2010 Sep;24(9):1737-47. Epub 2010 Jul 21. 10.1210/me.2010-0125 PubMed 20660297
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