p2xSTAT6-Luc2P
(Plasmid #35486)

• Depositing Lab: Axel Nohturfft
• Publication: pCMV-STAT6-IRES-Neo (unpublished)

Backbone

• Vector backbone: pGL4
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4267
• Modifications to backbone: Plasmid pGL4.32[luc2P/NF-κB-RE/Hygro] was modified by removing the hygromycin cassette as a 1782-bp XbaI fragment and by replacing the NF-κB promoter.
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: STAT6 promoter
• Promoter: 2x Stat6-c/EBP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: BglII (destroyed during cloning)
• 5′ sequencing primer: RVprimer3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Note: The design of the promoter insert is based on a reporter plasmid, TPU474, previously described by Mikita et al., 1996 [PMID 8816495]

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

KpnI--BglII fragment of precursor plasmid, containing an NFkappaB promoter, was replaced with a pair of annealed oligonucleotides containing two tandem copies of STAT6/cEBP binding sites, a composite element from the human germ line ε promoter.
Lab ID for this plasmid: pANG185.