TALE12
(Plasmid #35427)

• Depositing Lab: Feng Zhang
• Publication: Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. doi: 10.1038/nbt.1775

Backbone

• Vector backbone: pLenti
• Backbone size w/o insert (bp): 9204
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TALE12
• Alt name: dTALE12
• Species: Synthetic
• Insert Size (bp): 3531
• Promoter: EF-1a
• Tags / Fusion Proteins:
  • EGFP (C terminal on insert)
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: EF1a-fwd, zTALE-F
• 3′ sequencing primer: EGFP-N, zTALE-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that though this plasmid contains the sh_ble gene, the Zhang lab has not tested whether this plasmid confers Zeocin selection.