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Addgene

pCP5
(Plasmid #35397)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35397 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDW1714
  • Backbone manufacturer
    unknown
  • Backbone size (bp) 13844
  • Vector type
    Yeast Expression ; reporter
  • Promoter GDP promoter
  • Selectable markers
    TRP1, URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    Bacteria containing pCP5 grow fairly slowly - single colonies require ~16 hours to be visible on a plate. Grow on LB containing a maximum of 30mg/L Kanamycin.
  • Copy number
    High Copy

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloning nuclease targets into the BglII/SpeI sites of pCP5 removes the CcdB gene, thus allows negative selection against undigested backbone in common E. coli strains such as DH5-alpha.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCP5 was a gift from Daniel Voytas (Addgene plasmid # 35397 ; http://n2t.net/addgene:35397 ; RRID:Addgene_35397)

  • For your References section:

    Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218 10.1093/nar/gkr218 PubMed 21493687
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