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Addgene

pDZ264 (pKAN tdtomato cyc loxP KAN loxP)
(Plasmid #35193)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35193 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKAN
  • Backbone manufacturer
    David Miklos & Greg Freyer (Cold Spring Harbor Laboratory)
  • Backbone size w/o insert (bp) 4191

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tdtomato cyc loxP KAN loxP
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer M13rev
  • 3′ sequencing primer M13F20
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was designed to construct C-terminal fusions of a protein of interest with tdtomato

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDZ264 (pKAN tdtomato cyc loxP KAN loxP) was a gift from Robert Singer (Addgene plasmid # 35193 ; http://n2t.net/addgene:35193 ; RRID:Addgene_35193)

  • For your References section:

    Real-time observation of transcription initiation and elongation on an endogenous yeast gene. Larson DR, Zenklusen D, Wu B, Chao JA, Singer RH. Science. 2011 Apr 22;332(6028):475-8. 10.1126/science.1202142 PubMed 21512033
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