pDZ207 (loxP KAN loxP 24xPP7SL MDN1)
(Plasmid #35191)

• Depositing Lab: Robert Singer
• Publication: Larson et al Science. 2011 Apr 22;332(6028):475-8.

Backbone

• Vector backbone: pCR4
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3956
• Vector type: Bacterial Expression, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: loxP KAN loxP 24xPP7SL MDN1
• Species: S. cerevisiae (budding yeast)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was designed to construct mRNAs that contain multiple copies of an RNA sequence (the 24xPP7 stem-loops) that can be recognized by a chimeric RNA-binding protein fused to GFP, encoded in plasmid pDZ276 (Addgene plasmid #35194). See associated publication for detailed methods and use of this plasmid.

Please note that the LacZa-ccdB fusion in this plasmid is not functional and was originally present in the vector backbone to aid in screening for insert.

Addgene's sequencing results identified a 3 nucleotide deletion at bp# 543-545 when compared to the full plasmid sequence provided by the depositing laboratory. This deletion is not known to affect plasmid function.