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mCherry-Rab5DN(S34N)
(Plasmid #35139)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35139 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherry-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4722
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rab5a
  • Species
    H. sapiens (human)
  • Mutation
    Dominant Negative- S34N
  • Entrez Gene
    RAB5A (a.k.a. RAB5)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer mCherry-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-Rab5DN(S34N) was a gift from Sergio Grinstein (Addgene plasmid # 35139 ; http://n2t.net/addgene:35139 ; RRID:Addgene_35139)

  • For your References section:

    Recruitment of OCRL and Inpp5B to phagosomes by Rab5 and APPL1 depletes phosphoinositides and attenuates Akt signaling. Bohdanowicz M, Balkin DM, De Camilli P, Grinstein S. Mol Biol Cell. 2012 Jan;23(1):176-87. Epub 2011 Nov 9. 10.1091/mbc.E11-06-0489 PubMed 22072788
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