pBSFI-p110γ
(Plasmid
#34892)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 34892 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBSFI
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Backbone manufacturerAoki et al., PNAS. 1998 Dec 8;95(25):14950-5
- Backbone size w/o insert (bp) 2949
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Vector typeAdaptor plasmid
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep110γ
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Alt namePIK3CG
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SpeciesH. sapiens (human)
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Entrez GenePIK3CG (a.k.a. IMD97, PI3CG, PI3K, PI3Kgamma, PIK3, p110gamma, p120-PI3K)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site MluI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBSFI-p110γ was a gift from Peter Vogt (Addgene plasmid # 34892 ; http://n2t.net/addgene:34892 ; RRID:Addgene_34892) -
For your References section:
Oncogenic transformation induced by the p110beta, -gamma, and -delta isoforms of class I phosphoinositide 3-kinase. Kang S, Denley A, Vanhaesebroeck B, Vogt PK. Proc Natl Acad Sci U S A. 2006 Jan 31;103(5):1289-94. Epub 2006 Jan 23. 10.1073/pnas.0510772103 PubMed 16432180