POPFOP
(Plasmid #34849)

• Depositing Lab: Paul Sternberg
• Publication: Green et al Cell. 2008 Aug 22;134(4):646-56.

Backbone

• Vector backbone: pJIM20
• Backbone size w/o insert (bp): 8000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Mutated TCF binding sites
• Insert Size (bp): 70
• Promoter: pes-10 minimal promoter
• Tags / Fusion Proteins:
  • mCherry (C terminal on backbone)
  • let-858 3'UTR (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (unknown if destroyed)
• 3′ cloning site: AvrII (unknown if destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: EGFP-N, T3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

We also generated a control reporter, POPFOP; POP-1 Far from Optimal Promoter, that contains mutated Tcf/POP-1 binding sites. POPFOP was made by a similar strategy as POPTOP using mutated TCF binding sites from plasmid Super8xFOPflash (plasmid M51).