pAD12
(Plasmid #34832)

• Depositing Lab: Cynthia Kenyon
• Publication: Dillin et al Science. 2002 Oct 25;298(5594):830-4.

Backbone

• Vector backbone: pBluescript
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2961
• Modifications to backbone: T7 oligo was ligated into SacI/SpeI sites in pBluescript (SK+), thereby removing the XbaI, Not I, Sac II, BstX I sites.
• Vector type: Bacterial Expression, RNAi

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: SpeI (not destroyed)
• 5′ sequencing primer: M13F(-20)
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • pAD12 sequence
• A portion of this plasmid was derived from a plasmid made by: Andrew Dillin & Cynthia Kenyon, UCSF.
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Use HT115 bacteria for experiments--Grow O/N on LB supplemented with carbenicillin (100ug/ml) & tetracyclin (12.5ug/ml) and pick single colony for liquid culture.