pRK5-TGF beta type I receptor (mL45)
(Plasmid #34701)

• Depositing Lab: Ying Zhang
• Publication: Yu et al EMBO J. 2002 Jul 15;21(14):3749-59.

Backbone

• Vector backbone: pRK5 modified with flag
• Backbone manufacturer: Genentech
• Backbone size w/o insert (bp): 4700
• Modifications to backbone: Flag tag with stop codon at the end is inserted between SalI and HindIII.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TGF beta type I receptor
• Alt name: TGFb type I receptor
• Alt name: Tgfbr1
• Alt name: R4mL45
• Species: R. norvegicus (rat)
• Insert Size (bp): 1506
• Mutation: N265A, D267A, N268A
• Entrez Gene: Tgfbr1 (a.k.a. Alk5, Skr4, Tgfr-1, tbetaR-I)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This plasmid was derived from pRK5-TbRI-FLAG, Addgene plasmid #14831.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

3 amino acids, GSM, were introduced after aa148 for cloning purposes.