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Addgene

GST-PRMT1
(Plasmid #34699)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34699 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-2T
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4948
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRMT1
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Prmt1 (a.k.a. Hrmt1l2)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (unknown if destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer pGEX 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-PRMT1 was a gift from Steven Clarke (Addgene plasmid # 34699 ; http://n2t.net/addgene:34699 ; RRID:Addgene_34699)

  • For your References section:

    The predominant protein-arginine methyltransferase from Saccharomyces cerevisiae. Gary JD, Lin WJ, Yang MC, Herschman HR, Clarke S. J Biol Chem. 1996 May 24;271(21):12585-94. 10.1074/jbc.271.21.12585 PubMed 8647869
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