Stim1-CaRQ
(Plasmid
#34560)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 34560 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepTriEx-3
-
Backbone manufacturerPMID: 12864922
- Backbone size w/o insert (bp) 5000
-
Vector typeMammalian Expression, Bacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameStim1-CaRQ
-
SpeciesH. sapiens (human)
-
Insert Size (bp)3200
-
MutationQ63L in RhoA
-
Entrez GeneSTIM1 (a.k.a. D11S4896E, GOK, IMD10, STRMK, TAM, TAM1)
- Promoter CMV, T7
-
Tag
/ Fusion Protein
- YFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F, T7 (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byStim1 was cloned from Addgene plasmid 19754
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
MLCK peptide - KRRWKKNFIAVSAANRFKKI
IQ peptide - GSAITVQRYVRGIQARAYARFL
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Stim1-CaRQ was a gift from Kevin Truong (Addgene plasmid # 34560 ; http://n2t.net/addgene:34560 ; RRID:Addgene_34560) -
For your References section:
Ca2+-mediated synthetic biosystems offer protein design versatility, signal specificity, and pathway rewiring. Mills E, Truong K. Chem Biol. 2011 Dec 23;18(12):1611-9. 10.1016/j.chembiol.2011.09.014 PubMed 22195563