pMSCV-Atoh1wt-IRES-GFP
(Plasmid
#33333)
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Depositing Labs
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 33333 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMSCV-IRES-GFP
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Backbone manufacturerAddgene plasmid #33336
- Backbone size w/o insert (bp) 6575
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAtoh1
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SpeciesM. musculus (mouse)
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Insert Size (bp)1200
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Entrez GeneAtoh1 (a.k.a. Hath1, MATH-1, Math1, bHLHa14)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pLXSN 5' (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMSCV-Atoh1wt-IRES-GFP was a gift from Martine Roussel & Charles Sherr (Addgene plasmid # 33333 ; http://n2t.net/addgene:33333 ; RRID:Addgene_33333) -
For your References section:
Atoh1 inhibits neuronal differentiation and collaborates with Gli1 to generate medulloblastoma-initiating cells. Ayrault O, Zhao H, Zindy F, Qu C, Sherr CJ, Roussel MF. Cancer Res. 2010 Jul 1;70(13):5618-27. Epub 2010 Jun 1. 10.1158/0008-5472.CAN-09-3740 PubMed 20516124