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Addgene

pLVX-TIGHT-CYCLINF-Tet-OFF
(Plasmid #32977)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32977 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX-Tight-Puro
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 7791
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CYCLIN F
  • Species
    H. sapiens (human)
  • Entrez Gene
    CCNF (a.k.a. FBX1, FBXO1, FTDALS5)
  • Promoter P-TIGHT
  • Tags / Fusion Proteins
    • mCherry (N terminal on insert)
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (unknown if destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer LNCX_primer
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Backbone vector, pLVX-Tight-Puro map is attached.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-TIGHT-CYCLINF-Tet-OFF was a gift from Michele Pagano (Addgene plasmid # 32977 ; http://n2t.net/addgene:32977 ; RRID:Addgene_32977)
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