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Addgene

Mouse NHERF-1
(Plasmid #32950)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32950 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript SK1
  • Backbone manufacturer
    stratagene
  • Backbone size w/o insert (bp) 2960
  • Modifications to backbone
    none
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Slc9a3R1
  • Alt name
    NHERF
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1065
  • Mutation
    none
  • GenBank ID
    U74079
  • Entrez Gene
    Nherf1 (a.k.a. EBP-50, NHE-RF, NHERF-1, Slc9a3r1)
  • Promoter t3
  • Tag / Fusion Protein
    • none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer t3 primer
  • 3′ sequencing primer t7 primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

original clone from Stratagene's Mouse kidney cDNA library "UniZap" XR vector Cat # 93715

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mouse NHERF-1 was a gift from Edward Weinman (Addgene plasmid # 32950 ; http://n2t.net/addgene:32950 ; RRID:Addgene_32950)

  • For your References section:

    Molecular cloning of the cDNA and promoter sequences for the mouse sodium-hydrogen exchanger regulatory factor. Weinman EJ, Steplock D, Zhang X, Akhter S, Shenolikar S. Biochim Biophys Acta. 1999 Oct 6;1447(1):71-6. 10.1016/S0167-4781(99)00100-1 PubMed 10500246
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