pEGFP-mNFM
(Plasmid #32909)

• Purpose: Mammalian expression construct driving the expression of a fluorescent fusion protein consisting of EGFP linked to the N-terminus of mouse neurofilament protein M
• Depositing Lab: Anthony Brown
• Publication: Yan et al Cell Motil Cytoskeleton. 2007 Apr;64(4):299-309.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4041
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP-mouse NFM fusion
• Alt name: EGFP-mNFM
• Alt name: neurofilament protein M
• Species: M. musculus (mouse)
• Insert Size (bp): 3339
• GenBank ID:
• Entrez Gene: Nefm (a.k.a. NF-M, NF160, NF165, Nef3, Nfm)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: multiple primers
• 3′ sequencing primer: multiple primers

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid first described in Yan et al. (2007) "The Polypeptide Composition of Moving and Stationary Neurofilaments in Cultured Sympathetic Neurons" Cell Motility and the Cytoskeleton 64:299–309.

Mouse NFM cDNA sequence deposited in Genbank (Accession number DQ201636).