pEGFP-rNFM
(Plasmid #32907)

• Purpose: Mammalian expression construct driving the expression of a fluorescent fusion protein consisting of EGFP linked to the N-terminus of rat neurofilament protein M
• Depositing Lab: Anthony Brown
• Publication: Wang et al Nat Cell Biol. 2000 Mar;2(3):137-41.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4134
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP-rat NFM fusion
• Alt name: NFM
• Alt name: neurofilament protein M
• Species: R. norvegicus (rat)
• Insert Size (bp): 3330
• Entrez Gene: Nefm (a.k.a. Nef3, Nfm)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ApaI (not destroyed)
• 3′ cloning site: ApaI (destroyed during cloning)
• 5′ sequencing primer: multiple primers
• 3′ sequencing primer: multiple primers

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Rat NFM cDNA originally cloned by Ron Liem, Department of Pathology, Columbia University College of Physicians and Surgeons, New York
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid first described in Wang et al. (2000) "Rapid movement of axonal neurofilaments interrupted by prolonged pauses" Nature Cell Biology 2:137-141.