pEGFP-Sec24D
(Plasmid #32678)

• Depositing Lab: Henry Lester
• Publication: Richards et al J Biol Chem. 2011 Sep 9;286(36):31241-9. Epub 2011 Jul 18.

Backbone

• Vector backbone: pEYFP-C1
• Backbone manufacturer: BD Biosciences
• Backbone size w/o insert (bp): 4684
• Modifications to backbone: YFP was replaced with GFP
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sec24D
• Alt name: SEC24 family, member D
• Species: H. sapiens (human)
• Insert Size (bp): 3168
• GenBank ID: AF130464
• Entrez Gene: SEC24D (a.k.a. CLCRP2)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The original pEYFP-Sec24D construct was provided by Dr. David Stephens, Professor of Cell Biology, at the University of Bristol.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry