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Addgene

pISceI Dusp6:memGFP
(Plasmid #32668)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32668 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSceImemGFP
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Zebrafish Transgenesis

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Dusp6 promoter
  • Alt name
    Dusp6
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    10000
  • Entrez Gene
    dusp6 (a.k.a. Mkp3, wu:fd99d09)
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pISceI Dusp6:memGFP was a gift from Michael Tsang (Addgene plasmid # 32668 ; http://n2t.net/addgene:32668 ; RRID:Addgene_32668)

  • For your References section:

    The Rho kinase Rock2b establishes anteroposterior asymmetry of the ciliated Kupffer's vesicle in zebrafish. Wang G, Cadwallader AB, Jang DS, Tsang M, Yost HJ, Amack JD. Development. 2011 Jan;138(1):45-54. Epub 2010 Nov 23. 10.1242/dev.052985 PubMed 21098560
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