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Addgene

pCAG:GPI-GFP
(Plasmid #32601)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32601 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GPI-GFP
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer 5′ GFP-MYR-Eco (5′-CTT GAA TTC GCC ACC ATG GGA AGC AGC AAG AGC AAG CCA AAG GTG AGC AAG GGC GAG GAG CTG)
  • 3′ sequencing primer 3′-GFP primer (5′-GTC ATG AAT TCT TAC TTG TAC AGC TCG TCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG:GPI-GFP was a gift from Anna-Katerina Hadjantonakis (Addgene plasmid # 32601 ; http://n2t.net/addgene:32601 ; RRID:Addgene_32601)

  • For your References section:

    In vivo imaging and differential localization of lipid-modified GFP-variant fusions in embryonic stem cells and mice. Rhee JM, Pirity MK, Lackan CS, Long JZ, Kondoh G, Takeda J, Hadjantonakis AK. Genesis. 2006 Apr;44(4):202-18. 10.1002/dvg.20203 PubMed 16604528
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