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pEGFP-N1-CaMKKbeta S129A, S133A, S137A
(Plasmid #32454)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32454 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Modifications to backbone
    FLAG tag added into NheI site by cutting and inserting linker with FLAG tag and NheI overhangs. Second NheI site removed by site-directed mutagenesis.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Calcium/Calmodulin dependent protein kinase kinase beta
  • Alt name
    CaMKKbeta
  • Alt name
    CaMKK2
  • Species
    M. musculus (mouse)
  • Mutation
    S129A, S133A, S137A
  • GenBank ID
    NM_145358.1
  • Entrez Gene
    Camkk2 (a.k.a. 6330570N16Rik, AW061083, mKIAA0787)
  • Promoter CMV
  • Tags / Fusion Proteins
    • GFP (C terminal on backbone)
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A W374C mutation is also present in the pEGFP-N1-CaMKKbeta constructs that were used in this publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-N1-CaMKKbeta S129A, S133A, S137A was a gift from Anthony Means (Addgene plasmid # 32454 ; http://n2t.net/addgene:32454 ; RRID:Addgene_32454)

  • For your References section:

    Ca2+/Calmodulin-dependent Protein Kinase Kinase {beta} Is Regulated by Multisite Phosphorylation. Green MF, Scott JW, Steel R, Oakhill JS, Kemp BE, Means AR. J Biol Chem. 2011 Aug 12;286(32):28066-79. Epub 2011 Jun 13. 10.1074/jbc.M111.251504 PubMed 21669867
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