413-Met25 Kar2 TorsinADE E171Q GFP
(Plasmid
#32128)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32128 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbone413-Met25
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Vector typeYeast Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTorsinA
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SpeciesH. sapiens (human)
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Mutationdeletion of E at 302 or 303, E171Q
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Entrez GeneTOR1A (a.k.a. AMC5, DQ2, DYT1)
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Tags
/ Fusion Proteins
- Kar2 (N terminal on insert)
- GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer m13-r
- 3′ sequencing primer m13-f (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
413-Met25 Kar2 TorsinADE E171Q GFP was a gift from Susan Lindquist (Addgene plasmid # 32128 ; http://n2t.net/addgene:32128 ; RRID:Addgene_32128) -
For your References section:
TorsinA and the TorsinA-Interacting Protein Printor Have No Impact on Endoplasmic Reticulum Stress or Protein Trafficking in Yeast. Valastyan JS, Lindquist S. PLoS One. 2011;6(7):e22744. Epub 2011 Jul 26. 10.1371/journal.pone.0022744 PubMed 21818381