pGalT_EGFP-IRES_puro2b
(Plasmid #31804)

• Depositing Lab: Daniel Gerlich
• Publication: Guizetti et al Science. 2011 Mar 25;331(6024):1616-20. Epub 2011 Feb 10.

Backbone

• Vector backbone: EGFP_IRES_puro2b
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: pGALT
• Species: H. sapiens (human)
• Insert Size (bp): 242
• Mutation: *see comments

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

* Note that Addgene found a D76E point mutation in the insert during quality control sequencing. This mutation did not affect the original function of this construct (visualizing the Golgi structure), but if you plan on using the construct for something else, please take the mutation into account.