pQStrep2-ARPC3
(Plasmid #31580)

• Depositing Lab: Konrad Buessow
• Publication: Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Backbone

• Vector backbone: pQStrep2
• Backbone size w/o insert (bp): 3460
• Modifications to backbone: GenBank: AY028642
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Actin related protein 2/3 complex, subunit 3
• Alt name: PSFEp758G0811
• Species: H. sapiens (human)
• Insert Size (bp): 603
• GenBank ID: DQ000507
• Entrez Gene: ARPC3 (a.k.a. ARC21, p21-Arc)
• Tags / Fusion Proteins:
  • His (N terminal on backbone)
  • StrepII-tag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: TGAGCGGATAACAATTTCACACAG
• 3′ sequencing primer: GGCAACCGAGCGTTCTGAAC

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence.

Please note that Addgene's quality control sequence shows a small deletion in the vector region downstream of insert; the deletion does not affect any features in this construct.