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Addgene

pLS1
(Plasmid #31490)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31490 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEMBL9'
  • Backbone size w/o insert (bp) 4540
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lactose repressor protein
  • Alt name
    LacI
  • Insert Size (bp)
    3000
  • GenBank ID
    EG10525
  • Entrez Gene
    lacI (a.k.a. b0345, ECK0342)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site EcoR1 (unknown if destroyed)
  • 5′ sequencing primer CCAGCCAGCCAGACGCAG
  • 3′ sequencing primer CTGGGCGTGGAGCATCTG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Original LacI sequence from pIQ obtained from Joan Betz (see reference below to Hare and Sadler for generation of the pIQ
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Parent plasmid was pEMBL9' with insert of extended LacI sequence from pIQ (Hare & Sadler, Gene 3 (1978) 269-278.

For expression: E. coli BLIM cells (or any E. coli WITHOUT lactose repressor in the genome or accompanying plasmid)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLS1 was a gift from Kathleen Matthews (Addgene plasmid # 31490 ; http://n2t.net/addgene:31490 ; RRID:Addgene_31490)

  • For your References section:

    Plasticity of quaternary structure: twenty-two ways to form a LacI dimer. Swint-Kruse L, Elam CR, Lin JW, Wycuff DR, Shive Matthews K. Protein Sci. 2001 Feb . 10(2):262-76. 10.1110/ps.35801 PubMed 11266612
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