pLS1
(Plasmid #31490)

• Depositing Lab: Kathleen Matthews
• Publication: Swint-Kruse et al Protein Sci. 2001 Feb . 10(2):262-76.

Backbone

• Vector backbone: pEMBL9'
• Backbone size w/o insert (bp): 4540
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: lactose repressor protein
• Alt name: LacI
• Insert Size (bp): 3000
• GenBank ID: EG10525
• Entrez Gene: lacI (a.k.a. b0345, ECK0342)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: EcoR1 (unknown if destroyed)
• 5′ sequencing primer: CCAGCCAGCCAGACGCAG
• 3′ sequencing primer: CTGGGCGTGGAGCATCTG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Original LacI sequence from pIQ obtained from Joan Betz (see reference below to Hare and Sadler for generation of the pIQ
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Parent plasmid was pEMBL9' with insert of extended LacI sequence from pIQ (Hare & Sadler, Gene 3 (1978) 269-278.

For expression: E. coli BLIM cells (or any E. coli WITHOUT lactose repressor in the genome or accompanying plasmid)