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Addgene

pDOCKING-Neo
(Plasmid #31441)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31441 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCSGFP2
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression, Cre/Lox ; phiC31/attP
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ECFP-neomycin
  • Insert Size (bp)
    1600
  • Tag / Fusion Protein
    • ECFP (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Docking site for phiC31 mediated single copy integration. Additional loxP site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDOCKING-Neo was a gift from Gerhart Ryffel (Addgene plasmid # 31441 ; http://n2t.net/addgene:31441 ; RRID:Addgene_31441)

  • For your References section:

    Double conditional human embryonic kidney cell line based on FLP and PhiC31 mediated transgene integration. Waldner C, Rempel O, Schutte F, Yanik M, Solomentsew N, Ryffel GU. BMC Res Notes. 2011 Oct 18;4:420. 10.1186/1756-0500-4-420 PubMed 22008483
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