pET21-E. coli Initiation Factor 3 (IF3)
(Plasmid #31340)

• Depositing Lab: Linda Spremulli
• Publication: Koc et al J Biol Chem. 2002 Sep 20. 277(38):35541-9.

Backbone

• Vector backbone: pET21c
• Backbone size w/o insert (bp): 5400
• Vector type: Bacterial Expression
• Selectable markers: kanamycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: BL21(De3)pArg
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: E. coli Initiation Factor 3
• Alt name: infC
• Species: E. coli
• Insert Size (bp): 550
• GenBank ID: EG10506
• Tag / Fusion Protein:
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: vector

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Claudio Gualerzi, Dr. Roberto Spurio

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry