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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 31304 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4100
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Vector typeMammalian Expression, Cre/Lox ; FLP/FRT
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameECFP(loxP)(FRT)EYFP
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Insert Size (bp)2300
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (destroyed during cloning)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cre and FLP reporter: from blue fluorescence to yellow fluorescence
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LCMV:ECFP(loxP)(FRT)EYFP was a gift from Gerhart Ryffel (Addgene plasmid # 31304 ; http://n2t.net/addgene:31304 ; RRID:Addgene_31304) -
For your References section:
Tagging muscle cell lineages in development and tail regeneration using Cre recombinase in transgenic Xenopus. Ryffel GU, Werdien D, Turan G, Gerhards A, Goosses S, Senkel S. Nucleic Acids Res. 2003 Apr 15. 31(8):e44. 10.1093/nar/gng044 PubMed 12682379