pSCAC-69
(Plasmid #31241)

• Purpose: Mitochondrial localization of EGFP, for real-time imaging of mitochondria in transgenic zebrafish
• Depositing Lab: Seok-Yong Choi
• Publication: Kim et al Biotechniques. 2008 Sep . 45(3):331-4.

Backbone

• Vector backbone: T2AL200R150G
• Backbone size w/o insert (bp): 4000

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mitochondrial localization enhanced green fluorescent protein
• Alt name: MLS-EGFP
• Species: D. rerio (zebrafish)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HIndIII (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: EGFP-N

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry