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Addgene

pcDNA3.1-Myc-His RTN3A1
(Plasmid #31087)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31087 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1/myc-His(-)B (invitrogen)
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    reticulon 3
  • Alt name
    RTN3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    711
  • GenBank ID
    AF059524
  • Entrez Gene
    RTN3 (a.k.a. ASYIP, HAP, NSPL2, NSPLII, RTN3-A1)
  • Tag / Fusion Protein
    • Myc-His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-Myc-His RTN3A1 was a gift from Jimtong Horng (Addgene plasmid # 31087 ; http://n2t.net/addgene:31087 ; RRID:Addgene_31087)

  • For your References section:

    Reticulon 3 binds the 2C protein of enterovirus 71 and is required for viral replication. Tang WF, Yang SY, Wu BW, Jheng JR, Chen YL, Shih CH, Lin KH, Lai HC, Tang P, Horng JT. J Biol Chem. 2007 Feb 23. 282(8):5888-98. 10.1074/jbc.M611145200 PubMed 17182608
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