pET21c(+)-mtPheRS
(Plasmid #31080)

• Depositing Lab: Linda Spremulli
• Publication: Bullard et al J Mol Biol. 1999 May 14. 288(4):567-77.

Backbone

• Vector backbone: pET-21c(+)
• Backbone manufacturer: Novagen (EMD Biosciences)
• Backbone size w/o insert (bp): 5400
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: This plasmid was cotransformed with plasmid pArgU218 into BL21(DE3) E. coli by electroporation. pArgU218 allows for expression of genes with rare codons, such as AGA and AGG, which are not commonly used in E. coli. Please see the methods section of the associated publication for more details on expressing mtPheRS in E. coli.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mature form human mitochondrial phenylalanine-tRNA synthetase
• Alt name: PheRS
• Species: H. sapiens (human)
• Insert Size (bp): 1355
• Mutation: Includes coding sequence for the mature form. Nt 126-1481. Amino Acids 38-414
• GenBank ID: AF097441
• Entrez Gene: FARS2 (a.k.a. COXPD14, FARS1, HSPC320, PheRS, SPG77, mtPheRS)
• Promoter: T7
• Tag / Fusion Protein:
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry