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pET21c-IF3mt
(Plasmid #31078)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31078 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-21c
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression
  • Selectable markers
    kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    BL21 pArg.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mature form human mitochondrial translational initiation factor 3
  • Alt name
    MTIF3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    738
  • Mutation
    Includes coding sequence for the mature form. Amino acids 32-278.
  • Entrez Gene
    MTIF3 (a.k.a. IF3mt)
  • Tag / Fusion Protein
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21c-IF3mt was a gift from Linda Spremulli (Addgene plasmid # 31078 ; http://n2t.net/addgene:31078 ; RRID:Addgene_31078)

  • For your References section:

    Identification of mammalian mitochondrial translational initiation factor 3 and examination of its role in initiation complex formation with natural mRNAs. Koc EC, Spremulli LL. J Biol Chem. 2002 Sep 20. 277(38):35541-9. 10.1074/jbc.M202498200 PubMed 12095986
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