pET21c(+)-EFG1mt
(Plasmid #31076)

• Depositing Lab: Linda Spremulli
• Publication: Bhargava et al Protein Expr Purif. 2004 Oct . 37(2):368-76.

Backbone

• Vector backbone: pET-21c(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5400
• Vector type: Bacterial Expression
• Selectable markers: chloramphenicol

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: BL21(DE3)(RIL). The RIL plasmid provides the isoacceptor tRNAs for Arg, Ile, Leu and the Chloramphenicol resistance
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mature form human mitochondrial elongation factor G isoform 1
• Alt name: GFM1
• Species: H. sapiens (human)
• Insert Size (bp): 2145
• Mutation: Includes the coding sequence for the mature form. Amino acids 36-751.
• Entrez Gene: GFM1 (a.k.a. COXPD1, EFG, EFG1, EFGM, EGF1, GFM, hEFG1, mtEF-G1)
• Tag / Fusion Protein:
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry