pCFJ210
(Plasmid #30538)

• Depositing Lab: Erik Jorgensen
• Publication: Jorgensen unpublished mosSCI reagents (unpublished)

Backbone

• Vector backbone: pDESTR4-R3
• Backbone size w/o insert (bp): 4000
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: Requires growth in a strain resistant to ccdB (DB3.1, ccdB Survival, or F')
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ttTi4348 region
• Alt name: Cbr-unc-119(+)
• Species: C. elegans (nematode); C. briggsae
• Insert Size (bp): 5200

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Unpublished MosSCI reagents from the Jorgensen lab.
This vector targets the site ttTi4348 on Chr. I.
Use with strain EG6701 at the CGC.
Note - plasmid contains TWO M13F primer sites.