pGL3-pomMx1
(Plasmid #30536)

• Purpose: Reporter (luciferase) for interferon signalling in fish
• Depositing Labs: Bertrand Collet, Christopher Secombes
• Publication: Collet et al Dev Comp Immunol. 2004 Jun . 28(7-8):793-801.

Backbone

• Vector backbone: pGL3-Basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 6469
• Total vector size (bp): 7089
• Modifications to backbone: Backbone is pGL3-Basic from promega. A G418 resistance cassette was amplified from the pCMV-Script (Strategene) plasmid, inserted by blunt-end ligation at the filled in BamHI site of pGL3-Basic.
• Vector type: Mammalian Expression, Luciferase
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Growth instructions: There is a G418 resistance cassette giving Kanamycin resistance in prokaryotes and G418 resistance in eukaryotes.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: rainbow trout Mx 1 promoter
• Species: Oncorhynchus mykiss
• Insert Size (bp): 620
• GenBank ID: AF310254

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: RVP3
• 3′ sequencing primer: GLP2

Resource Information

• Supplemental Documents:
  • Oncorhynchus mykiss Mx1 gene, promoter and partial sequence Genbank file
• A portion of this plasmid was derived from a plasmid made by: The G418 resistance cassette originates from pCMV-Script (Stratagene).
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry