pSK ZO1
(Plasmid
#30316)
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Depositing Labs
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 30316 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSK+ Bluescript
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 2960
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Vector typesubcloning
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameZonula Occludens-1
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Alt nameTJP1
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SpeciesH. sapiens (human)
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Insert Size (bp)6656
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Entrez GeneTJP1 (a.k.a. ZO-1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Eco RI (not destroyed)
- 3′ cloning site Eco RI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSK ZO1 was a gift from James Anderson & Alan Fanning (Addgene plasmid # 30316 ; http://n2t.net/addgene:30316 ; RRID:Addgene_30316) -
For your References section:
The tight junction protein ZO-1 is homologous to the Drosophila discs-large tumor suppressor protein of septate junctions. Willott E, Balda MS, Fanning AS, Jameson B, Van Itallie C, Anderson JM. Proc Natl Acad Sci U S A. 1993 Aug 15. 90(16):7834-8. 10.1073/pnas.90.16.7834 PubMed 8395056