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Addgene

pGEX-MK2
(Plasmid #30187)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 30187 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-KG
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 3200
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MK2
  • Alt name
    Kcna2
  • Alt name
    kv1.2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2300
  • GenBank ID
    NM_008417
  • Entrez Gene
    Kcna2 (a.k.a. Akr6a4, Gm10672, Kca1-2, Kv1.2, Mk-2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site XbaI (destroyed during cloning)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-MK2 was a gift from Bruce Tempel (Addgene plasmid # 30187 ; http://n2t.net/addgene:30187 ; RRID:Addgene_30187)

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